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. Author manuscript; available in PMC: 2020 May 26.
Published in final edited form as: J Vis Exp. 2019 Oct 3;(152):10.3791/59977. doi: 10.3791/59977

Table 1:

Amounts of plasmid and PEI-max used for transfection.

Plasmid Size (bp) Amount per well (μg) Ratio
pLKO5.0 7700 0.9 2
psPAX2 10668 0.6 1
pMD2.G 5822 0.3 1
PEI-max (stock: 100 mg/mL) 5 μL/well