1/2 cc LO-DOSE INSULIN SYRINGE |
EXELINT |
26028 |
general supply |
293T cells |
ATCC |
CRL-3216™ |
Cell line |
BD Luer-Lok disposable syringes, 10 ml |
BD |
309604 |
general supply |
BD Microtainer blood collection tubes, K2EDTA added |
BD Bioscience |
365974 |
general supply |
BD Precisionglide needle, 18 G |
BD |
305195 |
general supply |
BD Precisionglide needle, 22 G |
BD |
305155 |
general supply |
Collagen from calf skin |
Sigma-Aldrich |
9007-34-5 |
general supply |
Corning Costar Ultra-Low Attachment Multiple Well Plate, 6 well |
Millipore Sigma |
CLS3471 |
general supply |
CRISPR/Cas9 knock-in mice |
The Jackson Laboratory |
028555 |
mouse |
DietGel 76A |
Clear H2O |
70-01-5022 |
general supply |
Dulbecco’s Modified Eagle’s Medium (DMEM) - high glucose |
Sigma Aldrich |
D6429 |
Medium |
eBioscience 1X RBC Lysis Buffer |
Thermo fisher Scientific |
00-4333-57 |
Solution |
Falcon 100 mm TC-Treated Cell Culture Dish |
Life Sciences |
353003 |
general supply |
Falcon 5 mL round bottom polystyrene test tube |
Life Sciences |
352054 |
general supply |
Falcon 50 mL Conical Centrifuge Tubes |
Fisher Scientific |
352098 |
general supply |
Falcon 6 Well Clear Flat Bottom TC-Treated Multiwell Cell Culture Plate |
Life Science |
353046 |
general supply |
Fisherbrand microhematocrit capillary tubes |
Thermo Fisher Scientific |
22-362566 |
general supply |
Fisherbrand sterile cell strainers, 70 μm |
Fisher Scientific |
22363548 |
general supply |
Fixation Buffer |
BD Bioscience |
554655 |
Solution |
Guide-it Compete sgRNA Screening Systems |
Clontech |
632636 |
Kit |
Isothesia (Isoflurane) solution |
Henry Schein |
29404 |
Solution |
Lenti-X qRT-PCR Titration Kit |
Takara |
631235 |
Kit |
Lineage Cell Depletion Kit, mouse |
Miltenyi Biotec |
130-090-858 |
Kit |
Millex-HV Syringe Filter Unit, 0.45 mm |
Millipore Sigma |
SLHV004SL |
general supply |
PBS pH7.4 (1X) |
Gibco |
10010023 |
Solution |
PEI MAX |
Polysciences |
24765-1 |
Solution |
Penicillin-Streptomycin Mixture |
Lonza |
17-602F |
Solution |
pLKO5.sgRNA.EFS.tRFP |
Addgene |
57823 |
Plasmid |
pMG2D |
Addgene |
12259 |
Plasmid |
Polybrene Infection/Transfection Reagent |
Sigma Aldrich |
TR-1003-G |
Solution |
Polypropylene Centrifuge Tubes |
BECKMAN COULTER |
326823 |
general supply |
psPAX2 |
Addgene |
12260 |
Plasmid |
RadDisk – Rodent Irradiator Disk |
Braintree Scientific |
IRD-P M |
general supply |
Recombinant Murine SCF |
Peprotech |
250-03 |
Solution |
Recombinant Murine TPO |
Peprotech |
315-14 |
Solution |
StemSpan SFEM |
STEMCELL Technologies |
09600 |
Solution |
TOPO TA cloning kit for sequencing with One Shot TOP10 Chemically Competent E. |
Thermo fisher Scientific |
K457501 |
Kit |
Zombie Aqua Fixable Viability Kit |
BioLegend |
423102 |
Solution |
APC-anti-mouse Ly6C (Clone AL-21) |
BD Biosciences |
560599 |
Antibodies |
APC-Cy7-anti-mouse CD45R (RA3-6B2) |
BD Biosciences |
552094 |
Antibodies |
BV711-anti-mouse CD3e (Clone 145-2C11) |
Biolegend |
100349 |
Antibodies |
PE-Cy7-anti-mouse CD115 (Clone AFS98) |
eBioscience |
25-1152-82 |
Antibodies |
PerCP-Cy5.5-anti-mouse Ly6G (Clone 1A8) |
BD Biosciences |
560602 |
Antibodies |
FITC-anti-mouse CD4 (Clone RM4-5) |
Invitrogen |
11-0042-85 |
Antibodies |
BV510-anti-mouse CD8a (Clone 53-6.7) |
Biolegend |
100752 |
Antibodies |