Table 2.
Overview of the validated target genes of miR-223.
| Gene | Full gene name | Function | Species | References |
|---|---|---|---|---|
| NFI-A | Nuclear Factor I A-type | Transcription factor | Human | (16) |
| LMO2 | LIM-only protein 2 | Erythropoeisis regulator | Human | (18, 19) |
| PARP-1 | poly(ADP-ribose) polymerase 1 | Regulator in cell death and NF-κB activity | Human | (35) |
| IKKα | IkB kinase α | Regulator of NF-κB pathway | Human | (36) |
| CUL1 | Cullin 1 | Protein degradation and ubiquitination | Human | (37) |
| TAB2 | TGF-β-activated kinase 1/MAP3K7 | Activation of MAP3K7 in the IL-1 signaling pathway | Human | (37) |
| NLRP3 | NLR family pyrin domain containing 3 | Pattern recognition receptor | Human | (24, 38, 39) |
| RhoB | Rho-related GTP-binding protein | Small signaling G protein | Human | (39) |
| CFTR | CF transmembrane conductance regulator | Chloride channel | Human | (40) |
| STAT3 | Signal transducer and activator of transcription 3 | Mediating anti- and pro-inflammatory responses | Human | (41) |
| CXCL2 | C-X-C Motif Chemokine Ligand 2 | Neutrophil chemo-attractant | Human | (42) |
| CCL3 | C-C Motif Chemokine Ligand 3 | Neutrophil chemo-attractant | Human | (42) |
| IL-6 | Interleukin-6 | Pro-inflammatory cytokine | Human | (42) |
| HDAC2 | Histone deacetylase 2 | Deacetylation of lysine | Human | (43) |
| Mef2c | Myocyte enhancer factor 2c | Promotes proliferation of myeloid progenitors | Human | (17) |
| IGF-1R | Insulin-like growth factor-1 receptor | Activates PI3K-Akt and mTOR signaling | Human | (17, 44–46) |
| TGFBR3 | Transforming growth factor beta receptor III | TGF-β signaling | Human | (47) |
| CDK2 | Cyclin-dependent kinase 2 | Cell cycle | Human | (45) |
| p53 | Tumor Protein P53 | Tumorsuppressor gene | Human | (48) |
| EPB41L3 | Erythrocyte Membrane Protein Band 4.1 Like 3 | Cell proliferation, cell-cell contact | Human | (49) |
| ICAM-1 | Intercellular adhesion molecule 1 | Role in leukocyte trafficking | Human | (50) |
miR-223 validated targets by luciferase assays. The luciferase assay determine whether a specific miRNA controls the expression of a potential target gene. The 3′UTR of the potential target gene is cloned into the 3′ region of a luciferase reporter plasmid and co-transfected with the predicted miRNA, leading to repression of luciferase gene expression if the miRNA directly binds to the 3′-UTR.