FIGURE 8.

Relative expression of Glyma.19g066800 and Glyma.19g069200 was measured by qRT-PCR in RNA extracted from roots of Hap1 and Hap2 accession plants inoculated with S. fredii HH103 wild-type or mutants HH103ΩNopD and HH103ΩTtsI. Uninoculated soybean plants were used as the control. The 2^–ΔΔCt method was used to calculate the relative expression levels of candidate genes. Error bars indicate the mean ± standard error of three replications. Significant differences were determined by t-tests, and ‘*’ indicated the significant differences (p ≤ 0.05) at the time point.