Fig. 3.

Reconstruction of the cardiac structure of a paraffin embedded heart on multiple length scales. (a) Slice of the tomographic reconstruction acquired by KB-illumination with an effective pixelsize of 3.25 µm. The coarse interior structure can be identified, but smaller details such as small vessels and sub-cellular features are below the resolution of the setup. The red box marks a ROI of a scan with the PB illumination which is shown in (b). Erythrocytes and a layer of cells of the endothelium can be identified at this magnification. (c) In order to image sub-cellular features such as myofibrils, a biopsy of the heart was scanned at high resolution with the WG optic. In this geometry, details such as a small vessels filled with erythrocytes, endothelial cells, and nuclei in between myofibrils become visible.