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. 2020 May 19;11:950. doi: 10.3389/fimmu.2020.00950

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Copyright © 2020 Filbey, Mehta, Meijlink, Pellefigues, Schmidt and Le Gros.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-CD25 depletion of H. bakeri- induced Tregs does not affect reduction of LN hyperplasia after DBP-FITC challenge. (A) Timeline of DBP-FITC topical application model including treatment with anti-CD25 or isotype control. (B) Frequency of FoxP3+ CD25+ cells within the CD4+ T cell compartment at day 7 in the ear dLN. (C) Total number of live CD45+ cells from the ear dLN at day 7. (D) Relative difference in ear thickness at day 7 after DBP-FITC challenge in mice with or without Hb infection, treated with anti-CD25 or an isotype control. A one-way ANOVA with Tuckey's multiple comparisons test was used to test statistical significance. Graphs represent data combined from 2 experiments and show mean ± SD. n.s, not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.