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. 2020 May 19;11:950. doi: 10.3389/fimmu.2020.00950

Figure 5.

Figure 5

Prior Hb infection limits influx of innate inflammatory cells after DBP-FITC challenge. (A) Total number of CD45+ cells and their frequency within all live cells in the skin tissue at day 7 (24 h after DBP-FITC challenge). (B) Frequency within CD45+ cells, and total number of neutrophils (Ly6G+) in the skin at day 7. (C). Immunofluorescent staining of ear sections taken at day 7. Ly6G+ neutrophils (pink), FITC (green) and DAPI (blue). Scale bar represents 100 um. (D) Frequency within CD45+ cells and total number of macrophages (CD64+), (E) monocytes (Ly6C+), (F) eosinophils (SiglecF+), and (G) CD4+ T cells in the skin at day 7. A one-way ANOVA with Tuckey's multiple comparisons test was used to test statistical significance. Graphs represent data combined from 2 experiments and show mean ± SD. n.s, not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.