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. 2020 May 15;8:309. doi: 10.3389/fcell.2020.00309

FIGURE 2.

FIGURE 2

Comparison of surface marker expressions between two variants of synergistic three-phase protocol, including simplified schemes of both variants. Both variants were tested using the CBIA-50 hiPSC line. The first variant is the one that is fully described in this article. Stage 2 lasts for only 1 day, whereas stage 3 lasts for 3 days. (A) KDR is expressed on the surface of 98% of cells, whereas CXCR4 is dimly expressed on 22% of cells by day 2 of differentiation. (B–D) KDR remains highly expressed during phase 3 of differentiation (94–97%), and other endothelial markers start to emerge by day 3 and gradually increase until they reach very high expression (90%–95%) by day 5 of differentiation. (B) Second variant of our protocol, in which both phases 2 and 3 last for 2 days. (E,F) Similar to the previous variant, KDR is expressed on 94% of cells on days 2 and 3. (G) By the end of phase 3 on day 5 of differentiation, the KDR expression is slightly reduced to 87%. More importantly, other endothelial markers, CD31, CD34, and CD144, have expression of only 28, 30, and 19%, respectively.