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. 2020 May 15;8:309. doi: 10.3389/fcell.2020.00309

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Copyright © 2020 Farkas, Simara, Rehakova, Veverkova and Koutna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative light microscopy photographs that show the entire process of differentiation from hPSCs to derived ECFCs. Magnification in all images is 40×. (A) hPSCs prior to single-cell seeding. (B) hPSCs 1 day after the single-cell seeding. (C) Primitive streak that formed from hPSCs after 1-day exposure to phase 1 medium. (D) Mesoderm that formed from primitive streak after 1-day exposure to phase 2 medium. (E) Endothelium that started to form from mesoderm after 1-day exposure to phase 3 medium. (F) Endothelium continuously formed after 2-day exposure to phase 3 medium. (G) Endothelium is fully formed and ready to be processed after 3-day exposure to phase 3 medium. (H) Derived ECFCs at the beginning of the first passage 1 day after seeding. (I) Confluent-derived ECFCs by the end of the first passage; confluence was mostly achieved 4 days after initial seeding.