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. 2020 May 15;8:309. doi: 10.3389/fcell.2020.00309

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Copyright © 2020 Farkas, Simara, Rehakova, Veverkova and Koutna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of mean endothelial surface marker expression levels between our synergistic three-phase protocol and two N2B27 + StemPro protocols (high VEGF + forskolin protocol and DAPT protocol). Mean and standard deviation values were obtained from biological duplicates of differentiation experiments using CBIA-37 and CBIA-50 cell lines on day 5 of differentiation. Analysis was conducted via flow cytometry. The mean expression of surface markers CD31, CD144, CD34, and KDR was (A) 90% to 96% for our protocol, (B) 49% to 63% for the high VEGF + forskolin protocol, and (C) 44% to 54% for the DAPT protocol. Variability in the surface marker expression was lower for our protocol than the other tested protocols, as shown by the standard deviations.