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. 2020 May 15;8:309. doi: 10.3389/fcell.2020.00309

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Copyright © 2020 Farkas, Simara, Rehakova, Veverkova and Koutna.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface marker expression of cells differentiated by synergistic three-phase protocol from multiple hPSC lines by day 5 of differentiation along with the mean and standard deviation values across all hPSC lines used. The hESC line used was H9 (WA09), and the six hiPSC lines used were CBIA-3, CBIA-50, CBIA-58, CBIA-19, CBIA-37, and CBIA-7. Analysis of surface markers CD31, CD34, CD144, and KDR was conducted via flow cytometry. Expression of these markers ranged between 75% and 97%. Mean expression of these markers was between 85 and 94%.