TABLE 2.

Description of basic properties of our synergistic three-phase differentiation protocol and other monolayer differentiation protocols cited in this article.

Protocol	Duration of differentiation	Primitive streak and mesoderm differentiated by separate media (precision of differentiation)	Derived cells tested for standard surface markers	Derived cells tested for advanced progenitor markers or properties	Derived cells tested by tube forming assay/LDL uptake	Number of hPSC lines tested (robustness)	Percentage of successfully differentiated cells/derived cells yield per pm35 (efficiency)	Precise single cell seeding density/media volumes defined (reproducibility and standardization)	Cytokines or media used require individualized dosage for different PSC lines (reproducibility and standardization)
Our synergistic three phase protocol (Farkas et al.)	5 days	Yes	CD31, CD34, CD144, KDR	Surface markers CXCR4, CD157	Yes/Yes	7	85–94% CD31, CD34, CD144, KDR/up to 6 million cells	Yes/Yes	No
Patsch et al., 2015	5 days	No	CD31, CD144, vWF	In vivo test on mice, transcriptome	Yes/Yes	4	70.1% CD144/up to 8 million cells	No/No	No
Sahara et al., 2014	5 days	No	CD31, CD34, CD144, KDR	PCR array, lack of CD14, single cell assay, proliferation assay	Yes/No	4	50% CD31 + CD144	No/No	No
Sriram et al., 2015	5 days	Yes	CD31, CD34, KDR	Surface markers CXCR4, NRP1, migration assay, in vivo test in mice	Yes/Yes	2 (only hESC, no hiPSC)	90–95% CD31, CD34, KDR	No/No	No
Prasain et al., 2014	12 days	No	CD31, CD144, KDR	Surface marker NRP1, colony forming test, in vivo test on mice	Yes/No	4	Illegible but sorting necessary/3750 cells	No/Yes	No
Park et al., 2010	10–15 days + sorting +12 days	No	CD31, CD34, CD105	Colony forming, in vivo test on mice	Yes/Yes	2	10–16% CD31 + CD34/not mentioned	No/No	Yes
Harding et al., 2017	4–10 days	No	CD31, CD144, CD34 (negative)	No	Yes/Yes	2	67.8% CD31+CD144-day 4/further manual separation	No/No	Yes
Orlova et al., 2014	10–11 days	No	CD31, CD105, CD73, CD144, KDR	In vivo test in zebra fish, gene expression	Yes/No	3	19.9%/not mentioned	No/Yes	No
Tatsumi et al., 2011	5 days	No	CD31, CD34, CD144, KDR	No	Yes/Yes	4	20%/1.2 million cells	No/No	No
Bao et al., 2015	5 days	No	CD31, CD34, CD144, vWF	No	Yes/Yes	6	24.45 %CD31, CD34/not mentioned	No/No	Yes

Table includes relevant properties such as duration, precision, robustness, efficiency, reproducibility and standardization of differentiation, tested surface markers, tested progenitor properties, LDL uptake, and tube assay. Table includes information from original articles written by their respective authors.