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. 2020 May 19;8:278. doi: 10.3389/fcell.2020.00278

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Copyright © 2020 Wang, Zhang, Xu, Yang, Xu, Liu, Li, Xie and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hsa-miR-6768-5p can bind to hsa_circ_0000848 in HTR-8 cells. (A) The binding site of hsa-miR-6768-5p on hsa_circ_0000848 and the mutant information for hsa_circ_0000848 in the dual luciferase activity assay. (B) Results of the dual luciferase activity assay. (C) Results of the anti-AGO2 immunoprecipitation (RIP) assay. hsa_circ_0000848 levels in purified RNA collected from products of anti-AGO2 or anti-IgG RIP assay and cell lysate before immunoprecipitation (input) was detected by RT-qPCR. *P < 0.05 using unpaired t-tests. wt, wild type; mut, mutant.