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IF study of cell proliferation (marker: Ki-67) in HT-29 spheroids with and without cetuximab treatment. (a) Typical IF images showing distribution of Ki-67 (green) and DAPI (blue) in spheroids treated or untreated (1 mg/mL) for 24 h or 72 h. (b) Statistical relative quantification of Ki-67 changes in spheroids following drug treatment. Data were normalized to DAPI intensities. Student’s t-test was used to test the statistical significance (n = 6, *p < 0.05).
