Table 2.

PCR and sequencing primers used to amplify the Helvella corium species aggregate and relevant outgroup taxa in the study.

Locus1	Forward primer sequence (5ʹ-3ʹ)	Reverse primer sequence (5ʹ-3ʹ)
hsp	H_hspf4: CRGGCATCCGGGTGACGTAAT	H_hspr4: AGGGKGTTGTCGACTCCGAGG
rpb2	H_rpb2r24: TCCACAATCTGCATCCCGATTC	H_rpb2f4: CCAGACATGGACAGAAGGTTGAG
tef	EF595F3: CGTGACTTCATCAAGAACATG	EF1160R3: CCGATCTTGTAGACGTCCTG
LSU	H_LSUf12: AGCGGAGGAAAGAAACCAAC	H_LSUr22: TCCCAACAGCTATGCTCCTAC
ITS	ITS55: GGAAGTAAAAGTCGTAACAAGG	ITS45: TCCTCCGCTTATTGATATGC
ITS2	ITS35: GCATCGATGAAGAACGCAGC	ITS45: TCCTCCGCTTATTGATATGC

¹LSU: 28S large subunit ribosomal RNA, domains D1/D2; rpb2: RNA polymerase II, second largest subunit; hsp: heat shock protein 90; tef: translation elongation factor 1-alpha; ITS: The internal transcribed spacer region (ITS1, 5.8S and ITS2).

²From Landeros et al. (2015), modified in Skrede et al. (2017).

³From (Kauserud & Schumacher 2001).

⁴From Skrede et al. (2017).

⁵From White et al. (1990).