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. Author manuscript; available in PMC: 2020 May 26.
Published in final edited form as: Nat Protoc. 2017 Feb 16;12(3):581–603. doi: 10.1038/nprot.2016.165

Table 2 |.

IDAA PCR primers

Primer Sequence (5´−3´) Purpose
Fwd AGCTGACCGGCAGCAAAATTGn19–24 Forward primer with locus specific sequence (n19–24) to amplify the nuclease target site and with an extension (in italics) having the same sequence as FamFwd primer.
Rev n19–24 Reverse primer with locus specific sequence (n19–24) to amplify the nuclease target site.
FamFwd 6-FAM-AGCTGACCGGCAGCAAAATTG Constant primer with same sequence as the extension of the Fwd primer and labelled with 6-FAM to render the amplicon fluorescent.