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. 2020 May 19;11:737. doi: 10.3389/fphar.2020.00737

Figure 4.

Figure 4

CCT020312 activated PERK/eIF2α/ATF4/CHOP signaling. (A, B) MDA-MB-453 cells (A) and CAL-148 cells (B) were treated with various of CCT020312 for 24 h, or with 8 and 10 μM CCT020312 for indicated time, then cells were collected to detect PERK, p-PERK, eIF2α, p-eIF2α, ATF4, and CHOP level using Western blotting. (C, D) MDA-MB-453 cells (C) and CAL-148 cells (D) were transfected with 2 μg of CHOP RNAi plasmid using Lipofectamine 3000 for 6 h. After overnight recovery, MDA-MB-453 and CAL-148 cells were treated with 8 and 10 μM CCT020312 for 24 h, then cells were collected to detect CHOP, cleaved PARP, and Bax level using Western blotting CCT020312 abbreviated as CCT.