Table 1.
Marker gene identity and qPCR primer sequences a
| Mouse Genes | Gene Name | Gene ID | FW Sequence | RW Sequence | HNF4a target* |
|---|---|---|---|---|---|
| INSR | Insulin receptor | NM_ 010568.2 | CCCCACCCTTTGAGTCTGAT | CGTTGTGCAGGTAATCCTCG | Y |
| MAP3K3 | Mitogen- activated protein kinase kinase kinase 3 | NM_011947.3 | TGAGTGCTCTGGAGTGTGAG | TGAGTGCTCTGGAGTGTGAG | Y |
| EP300 | E1A binding protein p300 | NM_177821.6 | AACAGCAGCTCAGCCATCTA | CAGGATCCGGAGTGGGAAAT | Y |
| HNF4a | Hepatocyte Nuclear Factor 4, alpha | NM_008261.2 | AGCCAACGATCACCAAGCAA | ACTGGTCCCTCGTGTCACAT | Y |
| TRAF6 | TNF receptor associate factor 6 | NM_009424 | CTCGGAGTGCCGTGTATGTA | ACACACACAACCCGATCGTA | Y |
| SIKE1 | Suppressor of IKBKE | NM_025679.3 | ACAAACCCATTCAGGGGTGG | AGCTTGCTCTACCCTCAGGA | |
| HSPA8 | Heat shock 70kDa Protein 8 | NC_000075.6 | GGGTTGCAGACTTTCTCCAGT | AAGGCTGAGGATGAGAAGC A | Y |
| PRKAB1 | Protein Kinase Activated Beta-1 | NM_031869 | CTTCCTTGTGTCCCTGCAGAT | TTCGGAGTGGAAGATGTCGG | |
| YWHAG | 14-3-3 gamma | NM_018871.3 | AGACGTGGCCCTGTATTTGG | TTGGCGTCATGTATGGCTGT | Y |
| SEPW1 | Selenoprotein W,1 | NM_009156.2 | GTGACAGTAGCCGGGAAGTT | TGATGGCGGTCACCAGTTTC | Y |
| ZMAT2 | Zinc Finger, Matrin-Type 2 | NM_025594.3 | AGTGCGAGTTTTCCCCTCTG | AGCCTGAATAACCCAGTGCC | Y |
| ALDOC | Aldolase C | NM_001303423. 1 | TGGACCGAGCTAATCAGAGGA | TGAGTGGGGCATGATGACA G | Y |
| FXYD6 | FXYD Domain Containing Ion Transport Regulator 6 | NM_022004.6 | GGTTGGTGTTTGCTGTGGTC | CCGCAGCGTTTGTAGTGATG | Y |
| IKBKE | Inhibitor of Kappa Light Polypeptide Gene Enhancer in B Cells, Kinase | NM_019777.3 | CCACTTGGAGTGCAGGAAGA | TTTCGGGCCTTGTACACACT | Y |
| Human Genes | Gene Name | Gene ID | FW Sequence | RW Sequence | |
| EP 300 | E1A binding protein p300 | NM_001429 | GCAGTGTGCCAAACCAGATG | GGGTTTGCCGGGGTACAATA | Y |
| HNF4a | Hepatocyte Nuclear Factor 4-alpha | NM_001287184 | AGGGATTAACTCTGCCCAGC | ACATCCTCCTCCTGCTGCTA | Y |
| CYP1B1 | Cytocrhome P450 family1,subfamil y b 1 | NM_000104 | AACAAGGACCTGACCAGCAG | CCCTGAAATCGCACTGGTGA | Y |
| CYP24A 1 | Cytochrome P450, family 24, subfamily A, polypeptide 1 | NM_001128915 | ACCCAAAGGAATTGTCCGCA | CAAAACGCGATGGGGAGTTC | |
| INSR | Insulin receptor | XM_006722744.1 | GTGACAGACTATTACGTCCCGT | CCATCTGGCTGCCTCTTTCT | Y |
| MAP3K3 | Mitogen- activated protein kinase kinase kinase 3 | NM_203351 | TTGGACAGGTCAGCAGACAG | GGTTCCACCTTTGACCCCTT | Y |
| HBGEF | Heparin binding EGF like growth factor | DQ480720 | TTGAGTGAGAAGAGCCAAGG G | AGGCCTCTTTCATCACAGGA C | Y |