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. 2020 May 18;14(1):82–95. doi: 10.1080/19336918.2020.1766308

Figure 4.

Figure 4.

CCND1 is a target of FGFR1. (a) Western blot and (b)qRT-PCR analyzes of CCND1 expression levels in H1581 cells treated with FGFR1 ligand bFGF or FGFR1 inhibitor AZD4547, (c) Western blot and (d)qRT-PCR analyzes of CCND1 expression levels in H1581 cells transfected with FGFR1 overexpression plasmid or siRNA, (e) Western blot analyzes of CCND1 expression levels in cytoplasm and nucleus in H1581 cells transfected with FGFR1 overexpression plasmid, (f) CO-IP analyzes of FGFR1 expression levels after incubated with anti-CCND1 (g) After transfection of CCND1 overexpression plasmid or siRNA of CCND1 for 24 h, the protein levels of AKT and MAPK signaling pathways were measured by western blot. (h) After transfection of si-FGFR1 or CCND1 plasmid or co-transfection of si-FGFR1 and CCND1 plasmid for 24 h, the protein levels of AKT and MAPK signaling pathways were measured by western blot. P values were calculated by Student t-test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.