Figure 6.

Si-CCND1 and FGFR1 inhibitor exhibited synergistic antitumor effects in vitro and in vivo. The experiments were conduct using H1581 cells (a) H1581 cells were transfected with 0.3 nM siRNA-NC or si-CCND1, incubating with AZD4547 in a serial dilution. After 72 hours, CCK8 was used to evaluate cellular proliferation. CI values were determined by non-linear regression methods at any given effect. (CI = 1, additivity; CI.1, antagonism; CI,1, synergy) (b) Representative images showing tumor formation in the nude mouse treated with siRNA-NC, AZD4547, si-CCND1 or si-CCND1 with AZD4547 for 3 weeks(combine).(C,D,E) H1581 (2 × 10⁶ cells) transfected with sh-CCND1 or LV-NC in a volume of 50 μL (PBS:Matrigel = 4:1) were injected into the left lung of 6-week-old male BCLB/C nude mice (n = 20). One week after injection, 5 mice injected with sh-CCND1 and 5mice injected with LV-NC were treated with FGFR1 inhibitor AZD4547 (12.5 mg/kg/d) randomly for 3 weeks. (c) Representative images showing tumor formation in the nude mice treated with shRNA-NC, AZD4547, sh-CCND1 or sh-CCND1 with AZD4547 for 3 weeks (d) Quantification of Represent EMT markers was measured by Immunofluorescence. (e) Survival curve for the mice in each treatment group evaluated. P values were calculated by Student t-test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.