(A,B) Flow cytometric analysis of Chd9 wild-type (WT, n = 3) and knockout (KO, n = 4) bone marrow (BM) stem cell and progenitor cells revealed expansion of short–term hematopoietic stem cells (red asterisk denotes p–value: 0.018, ST–HSC). Other BM hematopoietic stem cell and progenitor populations display no statistically significant differences. Data plotted as frequency of hematopoietic cells, mean ±SD, p-value calculated using two-tailed Student’s t–test. HSCP- hematopoietic stem cells and progenitors: Lin−(lineage negative), HSC (hematopoietic stem cells), MPP (multipotent progenitor cells), LT-HSC (long-term hematopoietic stem cells). Progenitors: IgM+ (B-cell progenitors), CMP (common myeloid precursor), GMP (granulocyte-monocyte progenitors), MEP (megakaryocyte-erythroid progenitors).