Table 7.
Behavior expressions | Days in relation to challenge | P-value | ||||||
---|---|---|---|---|---|---|---|---|
-12 | 1 | 2 | 3 | Total | TRT3 | DAY4 | TRT × DAY | |
Positive contacts: | ||||||||
CON | 1.96 c | 1.59 c | 5.95 a,b | 0.77 c | 2.22 | 0.142 | 0.038 | 0.025 |
PRO | 8.29 a | 2.02 b,c | 3.88 a,b,c | 2.13 b,c | 3.72 | |||
BUT | 2.28 b,c | 1.82 b,c | 1.42 c | 2.34 b,c | 1.96 | |||
Total | 3.72A | 1.82B | 3.50A,B | 1.66B | ||||
Negative contacts: | ||||||||
CON | 0.61 | 0.58 | 3.39 | 0.67 | 1.10 | 0.571 | 0.080 | 0.563 |
PRO | 0.86 | 0.06 | 2.04 | 1.02 | 0.81 | |||
BUT | 1.04 | 0.30 | 0.61 | 0.52 | 0.59 | |||
Total | 0.83 | 0.27 | 1.82 | 0.72 | ||||
Exploration: | ||||||||
CON | 2.13 | 2.69 | 4.49 | 1.42 | 2.56 B | 0.050 | 0.347 | 0.265 |
PRO | 8.94 | 2.56 | 4.93 | 5.71 | 5.29 A | |||
BUT | 4.41 | 3.65 | 1.72 | 2.19 | 2.89 A,B | |||
Total | 4.80 | 2.96 | 3.53 | 2.86 | ||||
Feeding: | ||||||||
CON | 11.49 | 26.63 | 28.30 | 25.00 | 22.28B | 0.003 | 0.030 | 0.850 |
PRO | 29.05 | 33.87 | 55.06 | 34.81 | 37.58A | |||
BUT | 19.45 | 20.52 | 30.47 | 19.18 | 22.18B | |||
Total | 19.27 B | 26.73 A,B | 37.09 A | 25.91 A,B | ||||
Others: | ||||||||
CON | 3.06 | 4.71 | 3.46 | 1.23 | 2.96 B | 0.039 | 0.192 | 0.966 |
PRO | 5.95 | 5.90 | 6.60 | 3.72 | 5.48 A | |||
BUT | 3.84 | 2.86 | 4.24 | 2.28 | 3.28 B | |||
Total | 4.20 | 4.41 | 4.67 | 2.28 | ||||
Lying laterally without contact: | ||||||||
CON | 0.86 | 1.00 | 0.81 | 1.06 | 0.92 | 0.821 | 0.448 | 0.967 |
PRO | 1.44 | 0.41 | 0.46 | 1.77 | 0.92 | |||
BUT | 1.08 | 0.37 | 0.21 | 1.02 | 0.61 | |||
Total | 1.12 | 0.56 | 0.46 | 1.25 | ||||
Lying ventrally without contact: | ||||||||
CON | 1.80 | 3.96 | 4.41 | 6.66 | 4.00 | 0.937 | 0.001 | 0.887 |
PRO | 2.02 | 1.99 | 2.92 | 9.80 | 3.69 | |||
BUT | 2.28 | 4.45 | 3.24 | 7.56 | 4.16 | |||
Total | 2.02 B | 3.39 B | 3.50 B | 7.95 A | ||||
Lying laterally with contact: | ||||||||
CON | 12.46 | 4.97 | 7.90 | 9.55 | 8.53 A | < 0.001 | 0.258 | 0.946 |
PRO | 3.88 | 2.37 | 0.64 | 1.00 | 1.77 B | |||
BUT | 13.03 | 4.93 | 9.00 | 12.18 | 9.49 A | |||
Total | 9.24 | 4.00 | 4.84 | 6.40 | ||||
Lying ventrally with contact: | ||||||||
CON | 31.47 | 38.81 | 25.30a | 24.21 | 29.70 A | 0.008 | 0.249 | 0.683 |
PRO | 10.11 | 21.44 | 5.52b | 21.90 | 13.76 B | |||
BUT | 17.64 | 29.92 | 26.52a | 30.69 | 25.91 A | |||
Total | 18.75 | 29.59 | 17.47 | 25.40 |
LSmeans within rows without common letters differ by the Means-Tukey adjustment test (P < 0.05).
LSmeans within columns without common letters differ by the Means-Tukey adjustment test (P < 0.05).
Values expressed are a proportion of total number of observations (active + inactive). For the statistical analysis, data were previously transformed using square root transformation. Drinking and walking behaviors are not shown, as they were not modified by any experimental treatment.
No differences were found between d –2 and –1 before the inoculation, so these data were pooled and named as –1.
Treatment effect. Treatments: CON, plain diet without additives; PRO, plain diet supplemented with 1 kg/t of Proporc (109 cfu/kg of feed of Bacillus licheniformis); BUT, plain diet supplemented with 3 kg/t of Gustor BP70 (2.1g of partially protected sodium butyrate salt/kg of feed).
Inoculation effect (measured as difference between days before (–1) and after (1, 2, 3) the challenge).