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. 2020 May 13;16(5):e1008500. doi: 10.1371/journal.ppat.1008500

Fig 6. A non-consensus -35 region is required for BvgA-regulated Pptx activity.

Fig 6

Luciferase activity of B. pertussis BP536 carrying ectopically integrated plasmids. Values for the empty pSS3967 control (V) and for promoter-lux fusion derivatives harboring the wild type (Pptx) and -35 substitution derivatives are presented. Strains were grown on BG agar at 37°C for 2 days and assayed as described in Materials and Methods. Values were normalized to wild-type Pptx and data from at least four assays were used in the calculation of means, standard deviations, as indicated by error bars, and statistical analysis by one-way ANOVA. Outcomes of the latter analysis are presented using the symbols: ns, P > 0.05; *, P ≤ 0.05; ****, P ≤ 0.0001.