Table 2.
Summary of Targeted Virulence Gene Analysis Results in Aeromonad Genomes
| Virulence-Associated Genes | RAST Annotation of Coding DNA Sequence (Length in Amino acids, AA) | % of Presence (%) | Present | Absent | Flanking Sequences |
|
|---|---|---|---|---|---|---|
| Upstream | Downstream | |||||
| aer/act | Hemolysin (481–505 AA) | 52 | Most phylogroups | Caviae and Media phylogroups | Hypothetical protein | Mostly hydroxymethyl pyrimidine phosphate synthase ThiC |
| alt | Putative lipase (791–819 AA) | 97 | Most phylogroups | A. fluvialis and A. lacus | Hypothetical protein | GlpG except in the Schubertii phylogroup |
| ast | Predicted exported alpha-N-acetylgalactosaminidase (628–637 AA) | 19 | Hydrophila, Salmonicida, and Veronii phylogroups | Most phylogroups | Putative pyridoxine 5′-phosphate synthase (or hypothetical protein or a putative aspartate amino transferase) | Putative tagatose 1,6 bi-phosphate aldolase |
| colAh | Microbial collagenase secreted (910–920 AA) | 89 | Most phylogroups | Variable | Putative O-succinyl acid-CoA ligase (or hypothetical protein) | SanA protein |
| rtxA | RtxA toxin (4439–4849 AA) | 14 | Hydrophila phylogroup | Most phylogroups | RTX toxin activating lysine acyltransferase | Variable |
| exoA | Putative exotoxin A precursor (639–640 AA) | 14 | < id="625" data-dummy="list" list-type="suimple">
|
Most phylogroups | Putative cyanate transporter protein CynX | Ribosomal large subunit pseudouridine synthase F |
| ascF | Cytoplasmic protein AscF (82–89 AA) | 33 | Most phylogroups | Caviae, Media, and Molluscorum phylogroups | AscE | AscG |
| ascG | AscG (114–118 AA) | AscF | AscH | |||
| ascV | AscV T3SS inner membrane channel (705–706 AA) | AscY chaperone protein | Variable | |||
| aexT | ADP-ribosyltransferase (451–476 AA) | 13 (38% of T3SS+ genomes) | A. veronii and A. salmonicida | Most phylogroups | Type 3 secretion chaperone protein | AexU in A. veronii and EAL domain |
| aexU | ADP-ribosyltransferase (446–514 AA) | 20 (62% of T3SS+ genomes) | Encheleia, Salmonicida, Hydrophila, and Veronii phylogroups | Schubertii, Molluscorum, Media, and Caviae phylogroups | Type 3 secretion chaperone protein (or AexT) | EAL domain or phenylalanine tRNA synthetase beta subunit |
| lafA | LafA flagellin protein (280–297 AA) | 50 | Most phylogroups | Variable | Variable | Putative LafB flagellar hook-associated protein |
| ser/ahe2 | Putative extracellular protease (624–634 AA) | 67 | Most phylogroups | < id="692" data-dummy="list" list-type="suimple">
|
Variable | Hypothetical protein |