Confocal microscopy of established biofilms treated with PBS (a, d, g, j), C-FE (b, e, h, k) or S-FE (c, f, I, l). a-f are Live/Dead staining of UAMS-1 biofilms, with green representing live bacteria and red representing dead bacteria. a-c are volumetric representations. d-f are representative two-dimensional views. Similarly, g-I are volumetric views of biofilms stained with wheat germ agglutinin to visualize the polysaccharide matrix of the biofilms volumetrically, compared to in representative two-dimensional images (j-l).