Figure 4. SKAP2 is not required for K. pneumoniae-stimulated degranulation nor phagocytosis.
(A–B) Levels of (A) total MMP-9, and (B) neutrophil elastase (ELA2) from cell-free supernatant from K. pneumoniae-infected WT and Skap2-/- lung homogenates were analyzed by ELISA. Data are compiled from 2 to 4 independent experiments with 2–3 mice/genotype/experiment. Each dot represents a mouse and bars represent geometric means. (C) WT and Skap2-/- DIV neutrophils were incubated with encapsulated (Kp), unencapsulated (ΔcpsB Kp) K. pneumoniae, or Yptb ΔyscNU. Percent phagocytosis was calculated as CFUbacteria with neutrophils and gentamicin/CFUbacteria without gentamicin treatment. (A–C) Data are compiled from at least 3 independent experiments performed in technical triplicate. Statistics represent mean ± SEM and were assessed using one-way ANOVA with Sidak’s post-test.