Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Danio rerio) | Et(krt4:EGFP)sqet20ET | Parinov et al., 2004 | sqET20; RRID:ZFIN_ZDB-ALT-070628-20 | |
Genetic reagent (Danio rerio) | Et(krt4:EGFP)sqet4ET | Parinov et al., 2004 | sgET4; RRID:ZFIN_ZDB-GENO-110323-1 | |
Genetic reagent (Danio rerio) |
Tg(atoh1a:
TdTomato)nns8 |
Wada et al., 2010 | nns8; RRID:ZFIN_ZDB-GENO-120530-1 | |
Genetic reagent (Danio rerio) | sox2-2a-sfGFPstl84 | Shin et al., 2014 | stl84; RRID:ZFIN_ZDB-GENO-150721-11 | |
Genetic reagent (Danio rerio) | Tg(brn3c:GAP43-GFP) s356t | Xiao et al., 2005 | S356t; RRID:ZFIN_ZDB-GENO-200218-3 | |
Genetic reagent (Danio rerio) | Tg(hsp70l:dkk1-GFP)w32 | Stoick-Cooper et al. (2007) | W32; RRID:ZFIN_ZDB-ALT-070403-1 | |
Genetic reagent (Danio rerio) | Tg(hsp70l:myc-notch1a;cryaa:Cerulean)fb12 | Zhao et al. (2014) | fb12; RRID:ZFIN_ZDB-ALT-140522-5 | |
Genetic reagent (Danio rerio) | Tg(hsp70:atoh1a)x20 | Millimaki et al. (2010) | X20; RRID:ZFIN_ZDB-GENO-110315-10 |
|
Genetic reagent (Danio rerio) | yapmw48 | Miesfeld et al., 2015 | Ms48; RRID:ZFIN_ZDB-ALT-160122-5 | |
Genetic reagent (Danio rerio) | Tg(hsp70:lin28a-P2Amcherry;cmlc:GFP)psi30 | this paper | Details in Fish strain information | |
Genetic reagent (Danio rerio) | Tg(hsp70:let7-P2AGFP;cryaa:Venus) | this paper | Details in Fish strain information | |
Genetic reagent (Danio rerio) | lin28apsi37 | this paper | Details in Fish strain information | |
Antibody | anti-sox2 (Rabbit, polyclonal) | Abcam | Cat# Ab97959; RRID:AB_2341193 | IF(1:200) |
Antibody | anti-yap (Rabbit, polyclonal) | CST | Cat# 4912, RRID:AB_2218911 | IF(1:200) |
Antibody | anti-taz (Rabbit, polyclonal) | Abcam | Cat# Ab84927; RRID:AB_1925489 | IF (1:200), WB (1:500) |
Antibody | anti-GFP (Mouse, monoclonal) | Invitrogen | Cat# A11120; RRID:AB_221568 | IF(1:500) |
Antibody | Anti-yap (Mouse, monoclonal) | Santa Cruz | Cat# sc-271134; RRID:AB_10612397 | WB (1:1000) |
Antibody | Anti-lin28a (Rabbit, polyclonal) | CST | Cat# 3978; RRID:AB_2297060 | WB (1:500) |
Antibody | Anti-lats (Rabbit, monoclonal) | CST | Cat# 3477; RRID:AB_2133513 | WB (1:500) |
Antibody | Anti-p-mob1 (Rabbit, monoclonal) |
CST | Cat# 8699; RRID:AB_11139998 | WB (1:500) |
Antibody | Anti-β-actin (Mouse, monoclonal) | Sigma | Cat# A1978; RRID:AB_476692 | WB (1:2000) |
Antibody | Anti-digoxingenin POD (sheep, polyclonal) | Roche | 11207733910; RRID:AB_514500 | 1:2000 |
Antibody | Anti-fluorescein POD(sheep, polyclonal) | Roche | 11426346910; RRID:AB_840257 | 1:2000 |
Chemical compound, drug | EdU | Carbosynth | NE08701 | 3.3 mM |
Chemical compound, drug | LY411575 | Santa Cruz | sc-364529 | 2 μM |
Chemical compound, drug | Neomycin sulfate | Sigma | N6386 | 300 μM |
Chemical compound, drug | Alexa Fluor-594 Azide | Thermo Fisher Scientific | N6386 | |
Chemical compound, drug | Verteporfin | Selleckchem | S1786 | 5 μM |
Chemical compound, drug | Copper sulfate | Sigma | 451657 | 50 μM |
Chemical compound, drug | cisplatin | Sigma | 33342 | 500 μM |
Commercial assay or kit | TSA-Cyanine 3 Reagent | PerkinElmer | SAT704A001EA | |
Commercial assay or kit | TSA-FITC Reagent | PerkinElmer | SAT704A001EA | |
Commercial assay or kit | dynabeads | Invitrogen | 10015D |