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. 2020 May 21;13:4507–4508. doi: 10.2147/OTT.S259765

Figure 1.

Figure 1

Downregulation ofmiR-486-5p enhanced the cytotoxic effect of 5-Fu in pancreatic cancer cells. (A) PANC-1 and (B) MiaPaCa-2 cells were transfected with 10 nMmiR-486-5p agonist or 10 nMmiR-486-5p antagonist for 48 hrs. RT-qPCR was used to detect the level ofmiR-486-5p in PANC-1 and MiaPaCa-2 cells. (C) PANC-1 and (D) MiaPaCa-2 cells were transfected with 10 nM miR-486-5p antagonist, and then exposed to 5-Fu for 48 hrs. Cell Counting Kit 8 assay was used to determine the cell viability. (E, F) PANC-1 cells were Transfected with 10 nMmiR-486-5p antagonist and then exposed to 5-Fu for 48 hrs. Meanwhile, PANC-1 cells were transfected with 10 nMmiR-486-5p antagonist or exposed to 5-Fu for 48 hrs, respectively. Relative fluorescence expressions were quantified by Ki67 and DAPI staining. *P<0.05, **P<0.01 vs NC group; ##P<0.01 vs 5-FU group.