Figure 4.

a) Representative images of embryos injected with RNPs assembled from non-caged SLC24A5 gRNA or caged SLC24A5–4U gRNA, and either kept in the dark or irradiated (365 nm) for 30 sec, 1 min, or 5 min at 1 hpf. Embryos were also irradiated at later timepoints (6 hpf and 10 hpf) for 5 min. Images were recorded at 48 hpf. Arrows point to the retina, demonstrating loss of pigmentation through successful editing of the slc24a5 locus (scale bar = 300 μm). b) Phenotype frequencies of the injected embryos at 48 hpf. The golden phenotype was determined based on the level of retinal pigmentation, with mild representing small patches of pigment loss, and strong representing a majority or complete retinal pigment loss.