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. 2019 Sep 16;22(3):494–503. doi: 10.1007/s11307-019-01429-z

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© The Author(s) 2020

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1. — Data processing steps to obtain a pH calibration curve. a, b The percentage water signal over different saturation frequencies, obtained from chemical samples made of distilled water and iopamidol: a the Gaussian filtered signal; and b spline filtered signal. c The percentage water signal from iopamidol for Lorentzian line shapes fitted to the data in b. A spline fitted curve is also shown in c for comparison with Lorentzian fitted curve. d The percentage difference signal (CEST ratio) between distilled water and iopamidol. e The Lorentzian spectra over different pH values. f The pH calibration curve based on a log₁₀ ratio of the two CEST signal amplitudes and the corresponding error bars.