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. 2020 May 26;10:8686. doi: 10.1038/s41598-020-65628-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exosome Treatment Regulates CAF Detachment and Spreading Profiles. CAFs were seeded overnight on FN-coated surfaces. Cells were treated with or without exosomes for 24 hours and detached using a centrifugal force-based assay. (A) Adherent fractions of fibroblasts treated with either population of exosomes were normalized to control groups. The dashed line indicates control group. Statistics calculated using Kruskal-Wallis test and were reported as mean +/− SEM. (B) To determine the fraction of cells spreading, CAFs were treated with exosomes for 24 hours, detached, and adhered on FN coated surfaces. The number of cells spreading over total number of cells at each time point was reported. (C–E) 10X images of calcein-labeled fibroblasts for each condition were taken to analyze cell spread profiles after 12 hours. Scale bar: 100 μm. (F) Fibroblast spread area and (G) shape factor were measured at the 12-hour time point (N = 3). Statistics calculated using Student’s t-test and reported as values +/− SEM. *p < 0.05, ***p < 0.001.