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. 2020 May 26;10(5):61. doi: 10.1038/s41408-020-0329-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Predicted VAF (x-axis) was calculated by multiplying the original sample VAF by the dilution factor. To account for differences in performance due to varying DNA quality of individual samples in the mix, predicted VAFs were normalized to mean Duplex depth of individual samples vs. the negative control DNA preparations. The y-axis represents observed Duplex VAF of each mutation in the mix. Error bars represent Wilson 95% confidence intervals. r² for observed vs. expected VAF = 0.93.