Fig. 4. TKI-R mutation detection in pre-relapse TKI-treated patients.

Duplex Sequencing (DS) VAF of relapse mutation (red) and BCR-ABL1 transcript % (blue) are plotted on the primary y-axis, along with mean Duplex depth (gray bars, secondary y-axis) for 12 total DNA samples from pre-relapse time points from six patients who relapsed with a TKI-R mutation. Sanger sequencing of relapse DNA identified T315I in four patients, and V299L and V338G in one patient each. A quantifiable pre-relapse BCR-ABL1 transcript was unavailable for patient 5. Duplex Sequencing did not identify V338G in relapse or pre-relapse DNA from patient 6.