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. 2020 May 26;11(5):400. doi: 10.1038/s41419-020-2611-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a HUWE1 depletion diminished DNA-PKcs Ser2056 phosphorylation. HUWE1 shRNA-depleted cells were treated with VP-16 and harvested at indicated timepoints, then DNA-PKcs Ser2056 phosphorylation or ATM Ser1981 phosphorylation was examined in western blotting. b UBA3 knockdown abolished DNA-PKcs Ser2056 phosphorylation. UBA3 siRNA-depleted HeLa cells were treated with VP-16 and harvested at indicated timepoints, then DNA-PKcs Ser2056 phosphorylation was examined in western blotting. c, d MLN4924 treatment diminished DNA-PKcs Ser2056 phosphorylation after VP-16 or IR treatment. HeLa cells were pretreated with 3 μm MLN4924 for 1 h and subjected to VP-16 treatment or different doses of IR treatment, then the cells were harvested followed by western blotting with DNA-PKcs Ser2056 antibody or indicated antibodies.