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. 2020 May 26;10:8721. doi: 10.1038/s41598-020-65567-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Production of IL-1 & IL-33 in differentiated air-liquid interface (ALI) cultures of primary airway epithelial cells. Primary airway epithelial cells (PAECs) from non-asthmatics (n = 5) and asthmatics (n = 10) were cultured at an air-liquid interface, RNA and supernatants were collected at Days (D) 1, 5, 11 and 20. (a) IL-1α (b) IL-1β and (c) IL-33 expression from PAECs are expressed as normalized to base pair reads. The concentration of (d) IL-1α & (e) IL-1β released from PAECs was measured by ELISA. Means ± SEM are shown. Exact P values indicated.