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. 2020 May 26;11:2631. doi: 10.1038/s41467-020-16284-8

Fig. 1. The mayfly C. dipterum.

Fig. 1

a Simplified arthropod phylogeny highlighting Pterygota insects (green rectangle). Ephemeroptera (mayflies, in orange) are within Paleoptera, the sister group to all other winged insects. Number of gene gains (in green) and core gene gains (in blue) is shown at the root of selected lineages. b C. dipterum freshly hatchling. c Late nymph. d Female adult. e Male adult. f Cartoons depicting organs and developmental stages used for the transcriptome profiling. In grey, embryonic stages used: 4 days post fertilization (dpf) stage: germ disc (2 replicates, Paired-End (PE) RNA samples); 6 dpf stage: segmentation (1 replicate, PE); 10 dpf stage: revolution (2 replicates, PE) and 14 dpf stage: pre-nymph (2 replicates, PE)); in orange, nymphal tissues: heads of three different nymphal stages (4 replicates young/early nymphs, 2 replicates mid-stage nymphs, 4 replicate late nymphs (2 female and 2 male), Single-End (SE) RNA samples), nymphal gut (×2, PE), nymphal Malpighian tubules (×2, PE), gills (×2, PE), wing pads (×2, PE); pale pink: adult muscle (×2, PE), ovaries (×2, PE), testes (×2, PE), female adult brain (×2, PE) and male and female adult heads (2× each, PE, see Supplementary Data 7 for sample details). White arrows in (d) and (e) highlight compound eyes present in both male and female, whereas the turbanate eye (white arrowhead) is only present in the male (e).