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. 2020 May 26;11:2631. doi: 10.1038/s41467-020-16284-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Mfuzz clusters showing high expression during embryonic, b nymphal and c adult stages (E4: 4 dpf embryo, E6: 6 dpf embryo, E10: 10 dpf embryo, E14: 14 dpf embryo, eN: early nymph head, mN: mid nymph head, lN: late nymph head, A: adult head). Plot numbers indicate the number of genes assigned to each of these clusters. Colour code reflects ‘Membership’ values calculated by Mfuzz, where magenta corresponds to high values and green to low values of Membership score. Histograms show enriched GO terms (determined with topGO R package). Each of them contains genes that are enriched in GO terms related to developmental processes, chitin and chemical stimuli perception and visual perception, respectively. Uncorrected P values shown in plots corresponded to two-sided Fisher’s exact tests (BP: biological process, CC: cellular component, MF: molecular function).