Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 26;11:2631. doi: 10.1038/s41467-020-16284-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a OBP phylogenetic reconstruction. C. dipterum genes are shown in green, E. danica in blue, C. splendens in brown and D. melanogaster in mustard. OBPs expressed specifically (according to tau values > 0.8; Supplementary Data 11) in nymphal heads are highlighted by pink squares, in gills in magenta and OBPs expressed both in heads and gills are shown in purple. b C. dipterum chemosensory families distributed across Mfuzz clusters. Clusters containing the largest amounts of chemoreceptors are no. 18, 12, 9 and 10 (E4: 4 dpf embryo, E6: 6 dpf embryo, E10: 10 dpf embryo, E14: 14 dpf embryo, eN: early nymph head, mN: mid nymph head, lN: late nymph head, A: adult head). Numbers in plots show the genes, and between parentheses, core genes for each cluster. c Heat map showing high levels of OBP expression across RNA-seq samples. Increased expression in nymphal head samples. An important fraction of OBP genes are highly expressed in gills (red square). d Spatial expression of OBP219 (green) in neural structures, marked by HRP staining (in red) in abdominal gills. e Spatial expression of OBP260 in abdominal gills. f–f′, OBP199 expression pattern in the gills (f′) co-localises with HRP-stained neural cells. g Detail of a cluster of cells expressing OBP219 (g″′) and marked by HRP (g″) in close contact with a trachea (highlighted by white asterisk). Nuclei are detected by 4′,6-diamidino-2-phenylindole (DAPI) staining (white arrowheads). Scale bars: 50 μm. Spatial expression of OBP genes (d–g″′) was assayed in at least two independent experiments with n > 20 gills each.