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. 2020 May 26;11:2631. doi: 10.1038/s41467-020-16284-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Heatmap showing the level of raw statistical significance, using upper-tail hypergeometric tests, of orthologous gene overlap between modules from C. dipterum (vertical) and Drosophila (horizontal) obtained by Weighted Gene Correlation Network Analysis (WGCNA). b Gene expression distributions (log10 cRPKMs) within the wing modules across each sample (a) for C. dipterum (left) and D. melanogaster (right). c Enriched Gene Ontology terms (BP: biological process) of the orthologous genes shared between C. dipterum and D. melanogaster wing and wing disc modules, using the wing disc module of D. melanogaster as background. d–f Wing phenotypes resulted from knockdowns of orthologous genes shared between C. dipterum and D. melanogaster wing and wing disc modules using wing-specific driver nub-Gal4. d, d′ nub-Gal4; UAS-GFP control fly (d) and control wing (d′). e nub-Gal4; UAS-RNAi-CG9662 flies show severe wing phenotypes. f nub-Gal4; UAS-RNAi-CG14812 show extra vein tissue phenotypes. g Heat map showing transcriptome datasets clustering. C. dipterum wing pads and gills samples cluster together. h Number of genes per each of second most tissues for 98 genes highly expressed in wing pads (n = 98). Gills are highlighted in blue.