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. 2020 May 26;11(5):398. doi: 10.1038/s41419-020-2592-z

Fig. 1. KLC4 was related to chemoresistance in vitro.

Fig. 1

a Cell viability was measured every 12 h after treatment with 10 μM cisplatin. b Death of cells treated with or without 10 μM cisplatin during 12 h intervals. c After cisplatin treatment for 24 h, the cells were fixed with 4% paraformaldehyde and immunostained using an antibody targeting γH2AX (DNA damage marker); DNA was visualized using DAPI staining. d Protein levels of KLC4, cleaved PARP, and active caspase-3 were determined using western blotting after cisplatin treatment. e–g Cells treated with etoposide, gefitinib, or Taxol, respectively. Twenty-four hours after the drug treatments, the cells were analyzed using cell viability assay, followed by ELISA.