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Comparison of PCR amplification and sequencing success rate from the traditional PCR and Sanger Sequencing approach and the MEBarcoding results from this study.
| Marker | # successful sequences from MEBarcoding | # successful sequences from Sanger sequencing | Average pairwise distance between MEBarcoding & Sanger |
|---|---|---|---|
| rbcL | 91/96 | 94 | 99.59% |
| matK | 58 | 71 | 99.3% |
| trnH-psbA | 83 | 88 | 97.56% |
| ITS | 64 | 67 | 95.57% |
When available, sequences from both approaches were compared and a pairwise identity score is provided.
