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. 2020 May 26;10:8685. doi: 10.1038/s41598-020-65518-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR verification and survival curve analysis. (a) Gene expression by qRT-PCR analysis. The fold changes were calculated by using the 2^−ΔΔCt method comparing the GaAs-treated group to the NC group. Data are expressed as the means ± S.D. from 3 biological repeats and 3 technical repeats. *P < 0.05 compared to control groups by Mann-Whitney test. (b) The potential correlation between the expression of dysregulated genes from RNA-seq and qRT-PCR verification was analysed using Spearman’s rank test. A P value less than 0.05 was considered statistically significant. (c) TCGA data from UALCAN demonstrated that high expression of HSP90AA1 predicted a significantly poor prognosis of lung adenocarcinoma (LUAD) patients (P < 0.05); (d) TCGA data from UALCAN demonstrated that high expression of RAC1 predicted a significantly poor prognosis of lung squamous cell carcinoma (LUSC) patients (P < 0.05); (e) TCGA data from UALCAN demonstrated that high expression of CDKN1A predicted a significantly poor prognosis of LUAD patients (P < 0.05).