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. 2020 May 26;11(5):397. doi: 10.1038/s41419-020-2601-2

Fig. 5. ISRIB and GSK relieve PERK-mediated translational repression with similar efficiencies.

Fig. 5

a l-Homopropargylglycine (HPG) pulse experiments were performed to measure translation rates of HEK293 cells treated for 5 h with 100 nM Thap either alone or in combination with 500 nM ISRIB, and 2 μM GSK or DMSO (control). Representative images of HPG incorporation (Alexa 488 fluorescence) and fluorescence quantification of individual cells. Representative experiment from two independent experiments; number of cells per condition: DMSO = 164, Chx = 160, Thap = 132, Thap + ISRIB = 154, and Thap + GSK = 163. b HPG metabolic labeling were performed in Ch-transfected neurons as described in a. Representative images of HPG incorporation (Alexa 488 fluorescence) and fluorescence quantification in treated individual neurons. Number of neurons per condition: DMSO = 39, Chx = 40, Thap = 39, Thap + ISRIB = 40, and Thap + GSK = 40 from two independent experiments, Kruskal–Wallis and Dunn’s post hoc test. All error bars indicate 95% confidence intervals (CI), (n.s.) non-significant, *p < 0.05, and ***p < 0.001.