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. 2020 May 26;10:8736. doi: 10.1038/s41598-020-65483-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Custom dual-imaging microscopy system schematic showing laser droplet detection components, and peripheral automation equipment. (B) Illustrative populations at the input (Cells), Detection zone, Keep and Waste outlets to define cell sorting performance parameters. Phase-contrast image of the microfluidic chip (channel borders are white) showing three inlets, the Y-junction and the electrodes for dielectrophoretic sorting. The width of the channel where detection takes place is 83 μm. (C) Complete chip design is shown with three inlets to the left and two outlets to the right. The 4 ports at the top are for the electrodes.