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. 2020 May 26;10:8736. doi: 10.1038/s41598-020-65483-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Probability of the number of cells in a droplet calculated for λ = 0.1 (red up triangle), 0.5 (blue down triangle) and 1 (white circle). Box plot of expected probability values calculated for each experiment using experiment specific red blood cell concentration (CC) and mean droplet volume (V) in Eq. 5. CC is calculated before the experiment by a haemocytometer, V is calculated after the experiment by averaging the volume of all droplets. The box plot is denoted ‘Expected’ values because the real CC value would be less due to cell sedimentation. The lines are joining data points for visual purposes. MATLAB’s default box plot is used, the red line shows the median, and the box denotes the 25^th and 75^th percentiles. The error bars (whiskers) extend to cover all data points. (B) Droplet volume recorded across different experiments carried out on multiple chips.