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. 2020 May 26;10:8712. doi: 10.1038/s41598-020-65566-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrotactic response of metastatic sublines to electric fields of physiological strength. (a) Parental 4T1 cells and cancer cells purified from metastatic sites in different organs, when cultured in very low density, didn’t show significant directional migration in EFs. (b) Cancer cells, in confluent culture, showed significant anodal galvanotaxis in EFs. Data are shown as mean ± SEM and compared using one-way ANOVA followed by Dunnett’s test. *p < 0.05, **p < 0.01 when compared with the no EF controls; ^#p < 0.05, ^##p < 0.01 compared with parental 4T1 cells of the same condition.