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. 2020 May 20;11:617. doi: 10.3389/fphar.2020.00617

Table 2.

Methods for determination of chemical components of P. edulis.

Chemical components Plant part Location and number of geno-type Better methods Major findings Reference
Polyphenol Pulp Brazil, yellow passion fruit QuEChERS method combined with C18 as d-SPE clean-up sorbent/UHPLC-MS-MS Quercetin, rutin, 4-hydroxybenzoic, chlorogenic, ferulic, vanillic, caffeic, trans-cinammic, and p-coumaric acids, and the most abundant phenolic components were quercetin and vanillic acid. Rotta et al., 2019
Volatile compounds Pulp Brazil, yellow passion fruit Dynamic headspace/GC-MS and GC-O analysis 64 volatile compounds, and mainly include esters, alcohols, terpenes, aldehydes, and ketones, and so forth. Janzantti et al., 2012
Carbohydrate Pulp France, passion fruit (unknown) Acid hydrolysis, NaOH (2 M), Fehling solution 13.70 g glucose equivalent/100 g Septembre-Malaterre et al., 2016
Carotenoid Pulp France, passion fruit (unknown) UV-vis spectrophotometry at 450 nm 3.83 mg β-carotene equivalent/100 g Septembre-Malaterre et al., 2016
Vitamin C Pulp France, passion fruit (unknown) 2,6-dichloro-phenol-indophenol titrimetric method 44.40 mg ascorbic acid equivalent/100 g Septembre-Malaterre et al., 2016
Polyphenol Pulp France, passion fruit (unknown) Folin-Ciocalteu assay 286.60 mg gallic acid equivalent/100 g Septembre-Malaterre et al., 2016
Flavonoid Pulp France, passion fruit (unknown) Colorimetric assay 70.10 mg quercetin equivalent/100 g Septembre-Malaterre et al., 2016
Polyphenol Fresh green leaves Sri Lanka, passion fruit (unknown) 70% (vol/vol) acetone/alkaline hydrolysis/HPLC The total soluble, insoluble-bound phenolic compounds and total flavonoid from the extracts were 511.20 mmol/g (gallic acid equivalent), 66.62 mmol/g (gallic acid equivalent), and 111.69 mmol/g (rutin equivalent). Gunathilake et al., 2018a
Polyphenol Seeds Brazil, yellow passion fruit 70% ethanol at 80°C for 30 min 31.20 mg/g (gallic acid equivalent), and the major component was piceatannol (36.80 mg/g). de Santana et al., 2017
Polyphenol Peel Brazil, yellow passion fruit Ultrasound-assisted or Pressurized solvent extraction (ethanol at 60:40)/LC-DAD-ESI-MS 4.67 mg/g (gallic acid equivalent). and mainly include orientin, orientin-7-O-glucoside, vitexin and isoorientin. de Souza et al., 2018
Polyphenol Ripe fruits Panama, passion fruit (unknown) Methanol 81 mg/100 g (Gallic acid equivalent) fresh weight Murillo et al., 2012
Oil Seeds Brazil, yellow passion fruit SE using n-hexane as solvent High tocopherol and fatty acid like palmitic, stearic, oleic, linoleic, α-Linolenic, behenic, caprylic, and aproic Pereira et al., 2019
Bound terpenoids Juice Australia, purple passion fruit Almond glycosidase hydrolysis with C18 isolates (MeOH elution), HRGC-MS 15 C13 norterpenoid aglycons, and the main terpenoids are 4-hydroxy-β-ionol, 4-oxo- β -ionol, 4-hydroxy-7,8-dihydro-β-ionol, 4-oxo-7,8-dihydro- β -ionol, 3-oxo-α-ionol, isomeric 3-oxoretro-α-ionols, 3-oxo-7,8-dihydro -α-ionol, 3-hydroxy-1,1, 6-trimethyl-1,2,3, 4-tetrahydronaphthalene, vomifoliol, and dehydrovomifoliol, and so forth. Winterhalter, 1990