FIGURE 5.

In vitro biofilm assay using C. albicans and C. parapsilosis early failing VP clinical isolates. Biofilms were seeded and grown for 48 h to mature, the resulting biofilms were quantified using the XTT assay which produces a red product that can be measured spectrophotometrically at 492 nm (higher cell number = higher absorbance). The C. albicans G-3065 clinical isolate makes bigger biofilms than the C. parapsilosis G10402 clinical isolate, and this biofilm formation is influenced by CO₂ concentration. The graph represents three independent experiments each containing triplicates, error bars denote Standard Deviation. Paired two-tail t-tests were carried out: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not significant.