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. 2020 May 20;8:482. doi: 10.3389/fbioe.2020.00482

FIGURE 2.

FIGURE 2

PDGF-BB induces thickening of 3D-PAH media tissues. (A–C) Representative 3D-reconstructed images of 3D-PAH media tissues cultured with platelet-derived growth factor (PDGF)-BB [PDGF-BB (+)] or without PDGF-BB [PDGF-BB (-)]. 3D-PAH tissues were generated using PASMCs from three different PAH patients #1, #2, and #3. Side views are shown to facilitate the comparison of thickness. (D–F) Quantification of the thickness of 3D-PAH media tissues as shown in (A–C), respectively. (G) Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of CCND1 expression in 3D-PAH media tissues cultured with or without PDGF-BB. n = 3 independently constructed 3D-PAH media tissues from PAH patient #1. (H) Representative images of 3D-PAH media tissues generated using PASMCs derived from PAH patient #1, cultured with or without PDGF-BB. 3D-PAH media tissues were stained for Ki67 (red). Nuclei were stained by SYTOX Green (green). Scale bars = 50 μm. (I) Quantification of Ki67-positive nuclear area as a percentage of the total nuclear area as shown in (H). In (D–G) and (I), n.s., not significant, *p < 0.05, and ****p < 0.0001, unpaired Student’s t-test with Welch’s correction.