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. 2020 May 20;8:482. doi: 10.3389/fbioe.2020.00482

FIGURE 3.

FIGURE 3

Imatinib inhibits the thickening of 3D-PAH media tissues induced by PDGF-BB via suppressing proliferation of PASMCs. (A–C) Representative 3D-reconstructed images of 3D-PAH media tissues cultured in the presence of PDGF-BB with or without imatinib treatment. 3D-PAH tissues were generated using PASMCs from three different PAH patients #1, #2, and #3. Side views are shown to facilitate the comparison of thickness. (D–F) Quantification of the thickness of 3D-PAH media tissues as shown in (A–C), respectively. (G) RT-qPCR analysis of CCND1 expression in 3D-PAH media tissues cultured in the presence of PDGF-BB with or without imatinib treatment. n = 3 independently constructed 3D-PAH media tissues from PAH patient #1. (H) Representative images of 3D-PAH media tissues generated using PASMCs derived from PAH patient #1, cultured in the presence of PDGF-BB with or without imatinib treatment. 3D-PAH media tissues were stained for Ki67 (red). Nuclei were stained by SYTOX Green (green). Scale bars = 50 μm. (I) Quantification of Ki67-positive nuclear area as a percentage of the total nuclear area as shown in (H). In (D–G), and (I), ∗∗p < 0.01, unpaired Student’s t-test with Welch’s correction.